CARD11

CARD11
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4JUP, 4LWD
Identifiers
Aliases	CARD11, BENTA, BIMP3, CARMA1, IMD11, PPBL, caspase recruitment domain family member 11, IMD11A
External IDs	OMIM: 607210; MGI: 1916978; HomoloGene: 13024; GeneCards: CARD11; OMA:CARD11 - orthologs
Gene location (Human) Chr. Chromosome 7 (human)[1] Band 7p22.2 Start 2,906,142 bp[1] End 3,043,867 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5|5 G2 Start 140,858,745 bp[2] End 140,986,337 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in lymph node spleen appendix blood superficial temporal artery parotid gland palpebral conjunctiva amniotic fluid gallbladder bone marrow Top expressed in spleen thymus blood ciliary body Paneth cell subcutaneous adipose tissue duodenum jejunum right lung lobe morula More reference expression data BioGPS n/a
Gene ontology Molecular function guanylate kinase activity protein binding CARD domain binding Cellular component cytoplasm cytosol membrane T cell receptor complex immunological synapse membrane raft extracellular exosome plasma membrane CBM complex Biological process regulation of apoptotic process positive regulation of cytokine production T cell costimulation stimulatory C-type lectin receptor signaling pathway Fc-epsilon receptor signaling pathway positive regulation of T cell activation positive regulation of B cell proliferation positive regulation of T cell proliferation regulation of immune response positive regulation of I-kappaB kinase/NF-kappaB signaling T cell receptor signaling pathway regulation of B cell differentiation thymic T cell selection regulation of T cell differentiation signal transduction GMP metabolic process positive regulation of NF-kappaB transcription factor activity GDP metabolic process immunoglobulin production B cell differentiation B cell proliferation T cell activation lymphocyte activation homeostasis of number of cells I-kappaB kinase/NF-kappaB signaling TORC1 signaling Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 84433 108723 Ensembl ENSG00000198286 ENSMUSG00000036526 UniProt Q9BXL7 Q8CIS0 RefSeq (mRNA) NM_032415 NM_001324281 NM_175362 RefSeq (protein) NP_001311210 NP_115791 NP_780571 Location (UCSC) Chr 7: 2.91 – 3.04 Mb Chr 5: 140.86 – 140.99 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Caspase recruitment domain-containing protein 11 also known as CARD-containing MAGUK protein 1 (Carma 1) is a protein in the CARD-CC protein family that in humans is encoded by the CARD11 gene.^[5][6][7] CARD 11 is a membrane associated protein that is found in various human tissues, including the thymus, spleen, liver, and peripheral blood leukocytes. Similarly, CARD 11 is also found in abundance in various lines of cancer cells.^[5]

Function

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). CARD11 (CARMA1) has a domain structure similar to that of CARD10 (CARMA3) and CARD14 (CARMA2) as a member of the CARD-CC family with a C terminal MAGUK domain (the so-called CARMA proteins). The CARD domain of proteins in the CARD-CC family have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of NF-κB activation by recruitment and activation of MALT1. When overexpressed in cells, this protein family activates NF-κB and induces the phosphorylation of BCL10.^[7]

CARD11 is critical for T cell and B cell function and is activated after T cell receptor or B cell receptor stimulation. After receptor stimulation, CARD11 is phosphorylated by PKC-θ (in T cells) or PKC-β (in B cells). The phosphorylation induces formation of filamentous CARD11 multimers that recruit BCL10 and MALT1, which in turn activates NF-κB. Loss of function mutations in CARD11 cause severe combined immunodeficiency (SCID) since the function of cells critical for adaptive immunity are disrupted.

Structure

The structure of CARD11 involves multiple domains that impact the protein's ability to activate BCL10 and NF-κB activity. CARD11 has a CARD domain, a serine-threonine rich region, is associated with the N-terminus, which is essential for NF-κB signaling activity. The region following the CARD domain is highly coiled. In deleting the CARD domain, all NF-κB signaling activity was prevented. The CARD domain on CARD11 interacts with the CARD domain on BCL10 to initiate the signaling pathway.^[5]

On the C-terminus of CARD11 there is the MAGUK domain that is associated with the cell membrane. This domain is often referred to as the inhibitory domain. Protein kinase C activates CARD11 by phosphorylating serine residues within the inhibitory domain.^[5][8]

Interactions

CARD11 has been shown to interact with BCL10.^[9] This interaction occurs between the CARD domain on BCL10 and the CARD domain on CARD11, and results in signal propagation and NF-κB activation.^[8]

References

External links

• Human CARD11 genome location and CARD11 gene details page in the UCSC Genome Browser.

Further reading