NME2

Protein-coding gene in the species Homo sapiens

NME2

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
3BBB, 3BBF, 1NSK, 3BBC, 1NUE

Identifiers

Aliases

NME2, NDKB, NDPK-B, NDPKB, NM23-H2, NM23B, PUF, NME/NM23 nucleoside diphosphate kinase 2

External IDs

OMIM: 156491; MGI: 97356; HomoloGene: 133879; GeneCards: NME2; OMA:NME2 - orthologs

Gene location (Human)

Chr.	Chromosome 17 (human)^[1]

Band	17q21.33	Start	51,165,435 bp^[1]
Band	17q21.33	End	51,171,744 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 11 (mouse)^[2]

Band	11\|11 D	Start	93,840,640 bp^[2]
Band	11\|11 D	End	93,847,085 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

left ovary

skin of abdomen

skin of leg

left coronary artery

canal of the cervix

mucosa of esophagus

mucosa of transverse colon

lactiferous gland

right ovary

body of pancreas

Top expressed in

right kidney

epiblast

pancreas

quadriceps femoris muscle

morula

human kidney

islet of Langerhans

muscle tissue

embryo

embryo

More reference expression data

BioGPS

n/a

Gene ontology
Molecular function	transferase activity DNA binding nucleotide binding protein histidine kinase activity DNA-binding transcription factor activity metal ion binding kinase activity protein binding ATP binding nucleoside diphosphate kinase activity protein serine/threonine kinase activity fatty acid binding intermediate filament binding enzyme binding transcription coactivator activity GDP binding G-quadruplex DNA binding
Cellular component	cytoplasm cytosol cell projection focal adhesion ruffle extracellular exosome nucleus cell periphery lamellipodium extracellular region secretory granule lumen ficolin-1-rich granule lumen intermediate filament mitochondrial membranes perinuclear region of cytoplasm
Biological process	UTP biosynthetic process peptidyl-histidine phosphorylation nucleobase-containing small molecule interconversion CTP biosynthetic process regulation of transcription, DNA-templated phosphorylation positive regulation of epithelial cell proliferation positive regulation of keratinocyte differentiation negative regulation of apoptotic process nucleotide metabolic process nucleoside diphosphate phosphorylation transcription, DNA-templated cell adhesion nucleoside triphosphate biosynthetic process integrin-mediated signaling pathway GTP biosynthetic process regulation of epidermis development positive regulation of transcription by RNA polymerase II neutrophil degranulation negative regulation of myeloid leukocyte differentiation positive regulation of neuron projection development cellular response to oxidative stress protein autophosphorylation protein complex oligomerization response to growth hormone cellular response to glucose stimulus cellular response to fatty acid adenylate cyclase-activating G protein-coupled receptor signaling pathway positive regulation of transcription, DNA-templated
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


4831


18103

Ensembl


ENSG00000243678


ENSMUSG00000020857

UniProt


P22392


Q01768

RefSeq (mRNA)


NM_002512 NM_001018137 NM_001018138 NM_001018139 NM_001198682


NM_001077529 NM_008705

RefSeq (protein)

NP_001018147 NP_001018148 NP_001018149 NP_001185611 NP_002503
NP_001018146


NP_001070997 NP_032731

Location (UCSC)

Chr 17: 51.17 – 51.17 Mb

Chr 11: 93.84 – 93.85 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Nucleoside diphosphate kinase B is an enzyme that in humans is encoded by the NME2 gene.^[5]^[6]

Function

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally occurring transcripts (NME1-NME2) which encode a fusion protein consisting of sequence sharing identity with each individual gene product.^[6]

Interactions

NME2 has been shown to interact with NME3^[7]^[8] and HERC5.^[9]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000243678 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000020857 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Stahl JA, Leone A, Rosengard AM, Porter L, King CR, Steeg PS (February 1991). "Identification of a second human nm23 gene, nm23-H2". Cancer Res. 51 (1): 445–9. PMID 1988104.
^ ^a ^b "Entrez Gene: NME2 non-metastatic cells 2, protein (NM23B) expressed in".
^ Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (September 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923.
^ Negroni A, Venturelli D, Tanno B, Amendola R, Ransac S, Cesi V, Calabretta B, Raschellà G (September 2000). "Neuroblastoma specific effects of DR-nm23 and its mutant forms on differentiation and apoptosis". Cell Death Differ. 7 (9): 843–50. doi:10.1038/sj.cdd.4400720. hdl:11380/695506. PMID 11042679.
^ Hochrainer K, Kroismayr R, Baranyi U, Binder BR, Lipp J (July 2008). "Highly homologous HERC proteins localize to endosomes and exhibit specific interactions with hPLIC and Nm23B". Cell. Mol. Life Sci. 65 (13): 2105–17. doi:10.1007/s00018-008-8148-5. PMC 11131906. PMID 18535780. S2CID 6897759.

Thakur RK, Kumar P, Halder K, Verma A, Kar A, Parent JL, Basundra R, Kumar A, Chowdhury S (2009). "Metastases suppressor NM23-H2 interaction with G-quadruplex DNA within c-MYC promoter nuclease hypersensitive element induces c-MYC expression". Nucleic Acids Res. 37 (1): 172–83. doi:10.1093/nar/gkn919. PMC 2615625. PMID 19033359.
Gilles AM, Presecan E, Vonica A, Lascu I (1991). "Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzyme". J. Biol. Chem. 266 (14): 8784–9. doi:10.1016/S0021-9258(18)31515-1. PMID 1851158.
Rosengard AM, Krutzsch HC, Shearn A, Biggs JR, Barker E, Margulies IM, King CR, Liotta LA, Steeg PS (1989). "Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development". Nature. 342 (6246): 177–80. Bibcode:1989Natur.342..177R. doi:10.1038/342177a0. PMID 2509941. S2CID 4340066.
Webb PA, Perisic O, Mendola CE, Backer JM, Williams RL (1995). "The crystal structure of a human nucleoside diphosphate kinase, NM23-H2". J. Mol. Biol. 251 (4): 574–87. doi:10.1006/jmbi.1995.0457. PMID 7658474.
Chandrasekharappa SC, Gross LA, King SE, Collins FS (1993). "The human NME2 gene lies within 18kb of NME1 in chromosome 17". Genes Chromosomes Cancer. 6 (4): 245–8. doi:10.1002/gcc.2870060411. PMID 7685630. S2CID 28992233.
Ji L, Arcinas M, Boxer LM (1995). "The transcription factor, Nm23H2, binds to and activates the translocated c-myc allele in Burkitt's lymphoma". J. Biol. Chem. 270 (22): 13392–8. doi:10.1074/jbc.270.22.13392. PMID 7768941.
MacDonald NJ, De la Rosa A, Benedict MA, Freije JM, Krutsch H, Steeg PS (1993). "A serine phosphorylation of Nm23, and not its nucleoside diphosphate kinase activity, correlates with suppression of tumor metastatic potential". J. Biol. Chem. 268 (34): 25780–9. doi:10.1016/S0021-9258(19)74458-5. PMID 8245015.
Backer JM, Mendola CE, Kovesdi I, Fairhurst JL, O'Hara B, Eddy RL, Shows TB, Mathew S, Murty VV, Chaganti RS (1993). "Chromosomal localization and nucleoside diphosphate kinase activity of human metastasis-suppressor genes NM23-1 and NM23-2". Oncogene. 8 (2): 497–502. PMID 8381224.
Postel EH, Berberich SJ, Flint SJ, Ferrone CA (1993). "Human c-myc transcription factor PuF identified as nm23-H2 nucleoside diphosphate kinase, a candidate suppressor of tumor metastasis". Science. 261 (5120): 478–80. Bibcode:1993Sci...261..478P. doi:10.1126/science.8392752. PMID 8392752.
Kelsell DP, Black DM, Solomon E, Spurr NK (1993). "Localization of a second NM23 gene, NME2, to chromosome 17q21-q22". Genomics. 17 (2): 522–4. doi:10.1006/geno.1993.1362. PMID 8406509.
Engel M, Véron M, Theisinger B, Lacombe ML, Seib T, Dooley S, Welter C (1995). "A novel serine/threonine-specific protein phosphotransferase activity of Nm23/nucleoside-diphosphate kinase". Eur. J. Biochem. 234 (1): 200–7. doi:10.1111/j.1432-1033.1995.200_c.x. PMID 8529641.
Guignard F, Markert M (1996). "The nucleoside diphosphate kinase of human neutrophils". Biochem. J. 316 ( Pt 1) (1): 233–8. doi:10.1042/bj3160233. PMC 1217327. PMID 8645210.
Moréra S, Lacombe ML, Xu Y, LeBras G, Janin J (1995). "X-ray structure of human nucleoside diphosphate kinase B complexed with GDP at 2 A resolution". Structure. 3 (12): 1307–14. doi:10.1016/S0969-2126(01)00268-4. PMID 8747457.
Paravicini G, Steinmayr M, André E, Becker-André M (1996). "The metastasis suppressor candidate nucleotide diphosphate kinase NM23 specifically interacts with members of the ROR/RZR nuclear orphan receptor subfamily". Biochem. Biophys. Res. Commun. 227 (1): 82–7. doi:10.1006/bbrc.1996.1471. PMID 8858107.
Nosaka K, Kawahara M, Masuda M, Satomi Y, Nishino H (1998). "Association of nucleoside diphosphate kinase nm23-H2 with human telomeres". Biochem. Biophys. Res. Commun. 243 (2): 342–8. doi:10.1006/bbrc.1997.8097. PMID 9480811.
Pinon VP, Millot G, Munier A, Vassy J, Linares-Cruz G, Capeau J, Calvo F, Lacombe ML (1999). "Cytoskeletal association of the A and B nucleoside diphosphate kinases of interphasic but not mitotic human carcinoma cell lines: specific nuclear localization of the B subunit". Exp. Cell Res. 246 (2): 355–67. doi:10.1006/excr.1998.4318. PMID 9925751.
Negroni A, Venturelli D, Tanno B, Amendola R, Ransac S, Cesi V, Calabretta B, Raschellà G (2000). "Neuroblastoma specific effects of DR-nm23 and its mutant forms on differentiation and apoptosis". Cell Death Differ. 7 (9): 843–50. doi:10.1038/sj.cdd.4400720. hdl:11380/695506. PMID 11042679.
Roymans D, Willems R, Vissenberg K, De Jonghe C, Grobben B, Claes P, Lascu I, Van Bockstaele D, Verbelen JP, Van Broeckhoven C, Slegers H (2000). "Nucleoside diphosphate kinase beta (Nm23-R1/NDPKbeta) is associated with intermediate filaments and becomes upregulated upon cAMP-induced differentiation of rat C6 glioma". Exp. Cell Res. 261 (1): 127–38. doi:10.1006/excr.2000.5037. PMID 11082283.
Baillat G, Gaillard S, Castets F, Monneron A (2002). "Interactions of phocein with nucleoside-diphosphate kinase, Eps15, and Dynamin I". J. Biol. Chem. 277 (21): 18961–6. doi:10.1074/jbc.M108818200. PMID 11872741.
Fournier HN, Dupé-Manet S, Bouvard D, Lacombe ML, Marie C, Block MR, Albiges-Rizo C (2002). "Integrin cytoplasmic domain-associated protein 1alpha (ICAP-1alpha ) interacts directly with the metastasis suppressor nm23-H2, and both proteins are targeted to newly formed cell adhesion sites upon integrin engagement". J. Biol. Chem. 277 (23): 20895–902. doi:10.1074/jbc.M200200200. PMID 11919189.

PDB gallery

1be4: NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM B FROM BOVINE RETINA
1bhn: NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA
1nsk: THE CRYSTAL STRUCTURE OF A HUMAN NUCLEOSIDE DIPHOSPHATE KINASE, NM23-H2
1nue: X-RAY STRUCTURE OF NM23 HUMAN NUCLEOSIDE DIPHOSPHATE KINASE B COMPLEXED WITH GDP AT 2 ANGSTROMS RESOLUTION